The aRTegen™ MTHFR 677 Real-Time PCR Kit is designed for the detection of the MTHFR c.677C>T (rs1801133) variant based on the real-time polymerase chain reaction (qPCR) principle, using specific primer sets and fluorescence-labeled hydrolysis (TaqMan) probes.
Cat No:
11230-25 (25 Rxn) , 11230-50 (50Rxn)
Key Technical Characteristics:
Probe-Based Allelic Discrimination: Each kit utilizes fluorescence hydrolysis probe technology for accurate differentiation of wild-type and variant alleles in real-time PCR applications, ensuring robust genotype calling.
Optimized Reaction Chemistry: Pre-formulated master mix reagents are provided to support consistent amplification efficiency and high analytical performance.
High Specificity and Sensitivity: Targeted probe design and optimized thermocycling parameters enable precise detection of single nucleotide changes with minimal cross-reactivity, supporting confident genotyping even in samples with low target abundance.
Internal Controls and Assay Validation: Each kit includes built-in amplification controls to monitor reaction integrity, contributing to reproducible performance and data reliability.
Easy-to-Use Format: Assay setup requires minimal preparation steps, facilitating efficient processing in routine testing environments.
Quality Management and Manufacturing Standards: Kits are manufactured under stringent quality systems appropriate for molecular diagnostic reagents, ensuring consistency between lots and adherence to performance specifications.
The aRTegen™ MTHFR 677 Real-Time PCR Kit is intended for research use only (RUO) and is not suitable for diagnostic purposes.
